Hopanes hyodeoxycholic bile pills and determination of CDCA.docVIP

 PAGE \* MERGEFORMAT 10 Hopanes hyodeoxycholic bile pills and determination of CDCA [Abstract] Objective To establish the hopane hyodeoxycholic bile pills, and the determination of CDCA. Methods HPLC-ELSD method used column was Diamonsil C18 (4.6 mm × 200 mm, 5 μm), mobile phase acetonitrile -0.1% acetic acid (50:50), flow rate 1.0 ml / min, column temperature is 30 ℃; evaporative light scattering detector drift tube temperature of 105 ℃, carrier gas flow rate 2.6 L / min. Results hyodeoxycholic In the range of 0.386 ~ 7.714 μg good linear relationship, r = 0.999 7, the average recovery was 94.7%, RSD = 2.8% (n = 6); chenodeoxycholic acid in the range of 0.406 ~ 8.128 μg showed a good line sexual relationship, r = 0.999 5, the average recovery was 92.3%, RSD = 1.1% (n = 6). Conclusion The method is simple, reliable, hopanes bile pills can be used for quality control. [Keywords:] hopane bile pills; HPLC-ELSD; hyodeoxycholic; CDCA Hopanes closing contained in the bile pills lt;”China Pharmacopoeiagt;” -2005 edition of the Department Ⅰ, from patchouli leaves and pig powder composed of two herbs, aromatic Huazhuo, heat Tongqiao effect, mainly for the wet muddy intrinsic, gallbladder Fire caused by the depressed nasal congestion, runny nose and forehead headache treatment. lt;”China Pharmacopoeiagt;gt; the original standard prescription by TLC scanning method hyodeoxycholic pig powder and chenodeoxycholic acid content of [ 1], because hyodeoxycholic and CDCA for the end of the absorption of UV absorption, using content was determined by TLC-scanning low sensitivity, the larger the error, and compared with HPLC, TLC itself complex with a complicated operation, and low detection limits less, so, we use high performance liquid chromatography with evaporative light scattering detector in the other in pink pig and pigs deoxycholic acid chenodeoxycholic acid content was determined to improve and enhance the preparation of quality control standards in order to bet