HPLC Determination of baicalin in LiYan mixture content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of baicalin in LiYan mixture content [Abstract] Liyan mixture can be determined to establish a baicalin content in high-performance liquid chromatography. [Methods] Hypersil-ODSC18 (250mm * 4.6mm 5μ m) column; mobile phase was methanol -1.5% phosphoric acid 10% methanol solution (40:60, v / v); detection wavelength 276nm; a flow rate of 1.0ml/min ; column temperature: 40 ℃ . [Results] baicalin in 25.74μ g * ml-1 ~ 514.8μ g * ml-1 within the scope showed a good linear relationship (r = 0.9999), the average recovery was 99.8%; RSD1.43%. [Conclusion] The high-performance liquid chromatography method is simple, rapid, accurate, and can be used as quality control of the preparation method. [Keywords:] Liyan mixture of baicalin high-performance liquid chromatography assay Determination of the Baicalin in Pharynx Preparation by HPLC Abstract: [Objective] It is aimed at discovering an effective way of HPLC to detect the content of baicalin in the pharynx preparation. [Method] The column of Hypersil  ODS C18: 250mm * 4.6mm 5μ m; the mobile phase: 1.5% orthophosphoric acid and 10% methane alcohol; the detection wavelength: 276nm; velocity of flow: 1.0 ml / min; temperature: 40 ℃ ; the content is measured in the way of external standard method of apex area. [Result] By this method, baicalin takes on an excellent linearly relation within the range of 25.74μ g * ml-1 ~ 514.8μ g * ml-1, average recoveries get up to 99.8% and RSD 1.43% respectively. [Conclusion] This way is convenient and rapid, accurate and reliable , able to be used as a method of quality control on such baicalin preparation. Keywords:: pharynx preparation; baicalin; HPLC; content detection Liyan mixture is my hospital according to ancient Chinese medicine and modern clinical use of square sum up the experience drawn from the parties, prescription mainly by skullcap, wood butterfly, forsythia and other traditional Chinese medicine, has a heat Liyan S