HPLC Determination of brain Ling Capsules Icariin.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination of brain Ling Capsules Icariin [Abstract] Objective To establish a HPLC method in the determination of brain Ling Capsules Icariin. Methods Diamonsil C18 (4.6mm * 200mm, 5μ m) column, mobile phase: acetonitrile - water (26:74), flow rate: 1.0ml/min, detection wavelength: 270nm. The results of icariin in the range of 0.0404 ~ 0.504μ g linear relationship between peak area within the well, r = 0.99998, the average recovery was 98.8%, RSD of 1.3% (n = 6). Conclusion This measurement is simple, accurate, reproducible, and separation, and can be used for brain Ling Capsules quality control. [Keywords:] HPLC [Abstract] Objective To establish a HPLC method for the determination of icariin in Naolingsu capsules.Method A Diamonsil C18 column (200mm * 4.6mm, 5μ m) was used with a mobile phase of acetonitrile-water (26:74). The flow rate was 1.0ml/min and the detection wavelength was 270nm. Results The linear range of icariin was 0.0404 ~ 0.504μ g (r = 0.99998). The average recovery was 98.8% with RSD1.3% (n = 6). Conclusion The determination method is simple, accurate and can be used for the quality control of Naolingsu capsules. [Keywords:] HPLC; icariin; Naolingsu capsule; determination Brain Ling Capsules for the ‘Ministry of Health standards for traditional Chinese medicine set prescription drug formulations eighth book’ containing relevant species [1], by Huang Jing, epimedium, Schisandra and other 15 Chinese herbal medicines, has a supplement qi and blood, soothe the nerves and other brain efficacy. For the treatment of neurasthenia, forgetfulness, insomnia, dizziness, palpitations, tired body weakness, physically weak spontaneous perspiration, impotence spermatorrhea embolism. None of the original standard assay methods, Fang Epimedium as the main Medicine Smell, its active ingredient is icariin. In order to effectively control the drug quality, this study used HPLC determination of icariin and establish quality contr