HPLC determination of berberine in the content of pills car.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of berberine in the content of pills car 【Abstract】 Objective high-performance liquid chromatography parking pills berberine. Methods The analytical column was Kromasil C18; to acetonitrile  0.05 mmol / ml potassium dihydrogen phosphate (28:72) as mobile phase; detection wavelength was 345 nm, external standard method. The results of berberine in between 0.3 ~ 0.75 μg showed a good linear relationship, r = 0.999 3, the average recovery was 99.42%, RSD = 1.9% (n = 7). Conclusion The method is accurate, good stability, can be used as the compound preparation of quality control. Keywords: Parking pill; berberine; high-performance liquid chromatography Abstract: ObjectiveAn HPLC method for the determination of berberine hydrochloride in Zhuche Pills was established. MethodsA Kromasil C18 column was used with acetonitrile  0.05mmol / L CH3CN (28:72). Content was detected at a wavelength of 345nm. ResultsThe linear range was 0.3 ~ 0.75 μg with correlation coefficient, r = 0.999 3. The average recovery rate was 99.42%, RSD = 1.9% (n = 7). The method was accurate and repeatable. ConclusionIt can be used for quality control of Zhuche Pills. Keywords:: Zhuche Pills; Berberine hydrochloride; HPLC Parking close pills contained in the “Chinese Pharmacopoeia” in 2005 edition of the Department Ⅰ is determined by Coptis, Angelica, Bao Jiang, Ejiao 4 Herbs composition of compound preparation, with Ziyin, Zhi Li’s role, commonly used in Jiu Li Shangyin, dysentery abdominal pain, tenesmus embolism. Berberine Berberine is the main ingredient, “Chinese Pharmacopoeia” for their determination by UV - visible spectrophotometry. In order to better control the drug quality, this paper Coptis HPLC method for the determination of berberine hydrochloride. An instrument and reagent Japan’s high-performance liquid chromatography system (Japan HITACHI series); KQ  100A ultrasonic cleaner (