HPLC Determination of Compound Camphor Cream chlorhexidine gluconate content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of Compound Camphor Cream chlorhexidine gluconate content [Abstract] Objective: HPLC Determination of Compound Camphor Cream chlorhexidine gluconate content. Methods: Kromasil-C18 (250.0 mm * 4.6 mm, 5 μ m) column, mobile phase was methanol - water - triethylamine (44:56:1) (with phosphoric acid to adjust pH value to 3.5 ± 0.1), flow rate 1.0 ml / min, detection wavelength 258 nm. Results: The linear range of 0.08 ~ 0.72 μ g, r = 0.999 9, the average recovery was 99.92%, RSD = 0.04%. Conclusion: This method is convenient, sensitive, accurate, reproducible and can control the drug quality. [Keywords:] HPLC; compound camphor cream; content [Abstract] Objective: A HPLC method for the determination of Chlorhexidine Gluconate in Compound camphor tincture.Methods: Kromasil-C18 (250 mm * 4.6 mm, 5 μ m) column was used with methanol-water-triethylamine (44:56:1) (abjusted pH to 3.5 ± 0.1 by phosphoric acid) as the mobile phase. The flow rate was 1.0 ml / min.The content was detected at wavelen of 258 nm. Results: The calibration curve was linear in the range of 0.08 ~ 0.72 μ g , r = 0.999 9. The average recovery rate was 99.92% and RSD was 0.04%. Conclusion: The method is accurate and reliable, suitable for the determination of compound camphor tincture. [Keywords:] HPLC; Compound camphor tincture; Determination We use HPLC Determination of Compound Camphor Cream chlorhexidine gluconate content of the results shows that the method is convenient, sensitive, accurate, reproducible and can control the quality of the drug are reported as follows: An instrument and reagent 1.1 Instrument LC-10ATVP pump, SPD-10AVP-type detector, SLASS-VP chromatography workstation. Shimadzu UV-2450 UV - visible spectrophotometer. HR-200 type one ten-thousandth scale. 1.2 reagents and test drug Chlorhexidine acetate reference substance (C22H30Cl2N10 * 2C2H4O2) [(Lot No.: 100,183 ~ 1,999,902) (for HPLC use)] by the Chinese pharmaceutical