HPLC determination of blood levels of catechin Tiaojing Tablets Central Plains.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of blood levels of catechin Tiaojing Tablets Central Plains Author: South Jinsong Sun Haitao Wu Yanli Gao Bo [Abstract] Objective blood using high performance liquid chromatography determination of protocatechuic acid content of the Central Plains Tiaojing film. Methods acetonitrile -0.2% phosphoric acid (four ninety-six) as the mobile phase. The detection wavelength was 260 nm, measured blood levels of catechins Tiaojing Tablets Central Plains. Results The linear range of 0.0165 ~ 0.165 μ g, r = 0.999 99; the average recovery was 99.56%, RSD% = 0.39%. Conclusion The experimental linear relationship was good, simple, accurate, specific and can be used as control of blood to regulate menstruation film quality of the original medicinal and pharmaceutical methods. [Keywords:] blood to regulate menstruation film; high-performance liquid chromatography; protocatechuic acid Abstract: ObjectiveTo establish a quality control method of Buxue Tiaojing Tablets.MethodsThe HPLC system of a Alltima HP C18 (4.6 mm ID * 250mm, 5 μ m) was used and a mixture of acetonitrile-2% phosphoric acid (95:5, volume ratio ) as the mobile phase. The detection wavelength of Protoca-technic acid was 260 nm. ResultsProtocatechuic acid showed good linearity (r = 0.999 99) within the range of 0.0165 ~ 0.165 μ g. The average recovery was 99.56%, RSD% was 0.39% . ConclusionThe method is simple, accurate and exclusive and it can be used for the quality control of Buxue Tiaojing Tablets. Keywords:: Buxue Tiaojing Tablets; HPLC; Protocatechuic acid Tiaojing blood film is from Millettia, donkey-hide gelatin, Kong Nim son, cinnamon, Codonopsis and other 18 Chinese herbal medicines, has a blood qi, regulate menstruation efficacy, for women, anemia, looking chlorosis, Chibai vaginal discharge, menstrual cramps, by the leakage, amenorrhea embolism. Prescribing the composition of its complex, Millettia for Fang Jun drug, its function attending the blood,