HPLC determination of the content of Prulifloxacin.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of the content of Prulifloxacin 【Abstract】 Objective To establish Prulifloxacin method for the determination. Methods using high performance liquid chromatography; Alltima C18 column (150mm × 4.6mm, 5μm); acetonitrile: 0.005mol / L sodium dihydrogen phosphate solution (take 0.005mol / L sodium dihydrogen phosphate solution 100ml, plus three ethylamine 0.2ml, with phosphoric acid to adjust pH value to 6.0) (40:60) as mobile phase; flow rate 1.0ml/min, detection wavelength 278nm. Results Prulifloxacin in the concentration range of 12.8 ~ 204.1μg/ml showed a good linear relationship, r = 0.9999. Conclusion The method is rapid, simple, accurate and are higher for assay of Prulifloxacin. Keywords: Prulifloxacin HPLC determination method Content determination of prulixacin by HPLC ABSTRACT Objective A high performance liquid chromatographic method for the content determination of prulixacin was developed. Method The HPLC was performed on a column of Alltima C18 (150mm × 4.6mm, 5μm), using the mobile phase of 0.005mol / L sodium dihydrogen phosphate ( added with 0.2ml triethylamine and adjusted to pH 6.0 by phosphoric acid): acetonitrile (60:40) at the detection wavelength of 278nm with the flow rate of 1.0ml/min. Results The calibration curve was linear in the range of 12.8 ~ 204.1 μg / ml (r = 0.9999) for prulixacin. Conclusion The method was simple, quick and accurate for content determination of prulixacin. KEY WORDS Prulixacin; Content determination; HPLC Prulifloxacin by the Japanese drug company and Meiji Seika co-developed by third-generation broad-spectrum fluoroquinolone antibacterial drugs against Gram-positive bacteria and gram-negative bacteria with broad-spectrum antibacterial activity against Pseudomonas aeruginosa The MIC90 for 0.39μg/ml, far more than other similar products, antimicrobial activity [1,2]. Prulifloxacin Determination of bulk drugs have been reported in