HPLC Determination of wavelength conversion and crocin -1 geniposide content.docVIP

 PAGE \* MERGEFORMAT 10 HPLC Determination of wavelength conversion and crocin -1 geniposide content Study: Fujian Wu, PENG, Zhou Yuchun, Huang Liyun [Abstract] Objective Determination of wavelength conversion by HPLC and Western medicine Gardenia Gardenia safflower and glycoside -1 HPLC. Methods column was Agilent Zorbax Eclipse XBD-C18, mobile phase of acetonitrile: water (gradient elution), flow was 1 ml / min, detection wavelength 238 nm (0 ~ 20 min; 440 nm (20 ~ 40 min, the column temperature was 30 , detection wavelength conversion by 20 min. Results Geniposide in the range of 4.52 ~ 180.8 g, r = 0.999 9, crocin -1 in the range of 0.9 ~ 9.0 g, r = 0.999 7, and the chromatographic peak area was linear. The average recoveries were 99.52%, 100.76%, RSD 1.10%, respectively, 1.41%. Conclusion The method is simple, stable and accurate reproducibility. [Key Words] high performance liquid chromatography, geniposide, crocin -1 Abstract: ObjectiveTo determine the jasminoidin and safflower glucoside-1 of Gardenia. MethodsUsing RE-HPLC with Agient Zorbax Eclipse XBD-C18 column, the mobile phase was composed of a mixture of acetonitrile-water (grads elution). The flow rate was 1ml / min, and the UV detection wavelengths were set at 238nm (0 ~ 20 min) and 440nm (20 ~ 40 min), and the tempetature was at 30 . The wavelength was converted at 20min.ResultsIrisflorentins of the drugs showed linear relationship with peak areas in the range of 4.52 ~ 180 g / ml and 0.9 ~ 9.0g/ml respectively (r = 0.999 9,0.999 7). The average recoveries were 99.52% and 100.76%. The RSD were 1.10% and 1.41%. ConclusionThe method is simple, stable, accurate and repeatable. Keywords:: HPLC, Jasminoidin, Dafflower Glucoside-1 Modern research shows that many of gardenia contains the chemical composition, mainly iridoid class (class geniposide), pigments (crocin class), also contain flavonoids (Gardenia element type), organic esters (chlorogenic acid), D-mannitol, sterols, trite