HT1 type of tissue microarray instrument and application.docVIP

 PAGE \* MERGEFORMAT 17 HT1 type of tissue microarray instrument and application Of: Yang Jun, Su Baoshan, Zhao Shiping, Lei Qiang [Abstract] Objective To develop a simple, fast, easy to use tissue microarray instrument, verify its performance. Methods Preparation of tissue microarray design principles HT 1 type of tissue microarray instrument, the tissue microarray instrument array of holes by the recipient paraffin block shaper, the organization dig take control, vacuum apparatus and operating support embedded components. using a molding technique, only one operation can be quickly prepared an array of holes required for recipient paraffin block, using vacuum-embedded technology, both the precise positioning of tissue samples and fixed at the same level, but also the rapid extraction of tissue samples and the recipient paraffin block array of holes between the residual air bubbles. Results of tissue microarray using the HT 1-type device, can be prepared in a few seconds of high-quality array of holes within the recipient paraffin block, building a capacity tissue samples for the 56 cases of paraffin TMAs only less than 20min, and tissue samples arranged in the rules, no floating, lodging and shift phenomenon, and no air bubbles left. by HE and immunohistochemical staining confirmed that the arrangement of tissue microarray and tissue samples rules, structural integrity, a clear background, off-chip low (lt;1.0% + -1.1%). Conclusions HT 1 type of tissue microarray instrument is a simple structure, easy to use, easy to operate cost-effective tissue microarray instrument. [Keywords:] biochip, tissue arrays (tissue microarray), tissue array instrument ABSTRACT: Objective To design and develop a reliable, ease to use and cheap tissue microarryer for making tissue microarrays, and discuss its characteristics. Methods According to the facture procedure and principle of tissue microarray construction, HT 1 tissue microarrayer was designed and developed. T