Human bone marrow mesenchymal stem cells into hepatocyte-like cells induced to differentiate.docVIP

 PAGE \* MERGEFORMAT 18 Human bone marrow mesenchymal stem cells into hepatocyte-like cells induced to differentiate [Abstract] Objective: To study the human bone marrow mesenchymal stem cells (human bone marrow derived mesenchymal stem cells, hBM-MSCs) in the hepatocyte growth factor (HGF), fibroblast growth factor -4 (FGF-4), suppression oncostatin M (OSM) induced in vitro to induce differentiation of liver cells. Methods: Density gradient centrifugation and adherent culture obtained hBM-MSCs, isolated hBM-MSCs spread 6th generation amplification, flow cytometry hBM-MSCs detected surface CD34, CD45, CD105, CD166 expression. hBM-MSCs 6 on behalf of the students up to 100% confluence two-step method hBM-MSCs were induced to differentiate. 1 to 14 days induction medium with IMDM (containing 2% FCS, 20 ng / mL HGF, 20 ng / mL FGF-4), 15 ~ 21 days in the medium plus OSM (10 ng / mL), to promote the induction of cell maturation, while collecting the first 0, 7, 14, 21 days of culture supernatant samples, ELISA method to measure the concentration of ALB. 21 d after the induction using RT-PCR, immunofluorescence detection of markers of liver cells after the induction of CK18, ALB expression, PAS MSCs induced glycogen storage staining. Results: spread to the first 6 generations hBM-MSCs CD34, CD45 negative expression, CD105, CD166 positive expression. in the role of cytokines, and after 21 d in vitro, hBM-MSCs morphogenetic changes into a polygon by a long fusiform, round-like, and liver cells with specific phenotypic and functional. RT-PCR detected CK18, ALB expression can be detected by immunofluorescence after induction of intracellular CK18, ALB, cultivation clear in the secretion of ALB and after induction of liver glycogen storage within cells. Conclusion: hBM-MSCs in vitro can be converted into liver function and liver-like cells. [Keywords:] Mesenchymal stem cells; human bone marrow mesenchymal stem cells; liver cells; cell factor Abstract: Objective: