Human bone morphogenetic protein adenoviral vector7.docVIP

 PAGE \* MERGEFORMAT 10 Human bone morphogenetic protein adenoviral vector7 Of: Lixin You, Wang Jintang, Liu Miao, Wang Quanying, YANG Guang laugh, Li Meng [Abstract] Objective To study the human bone morphogenetic protein-7 (hBMP7) into the treatment of bone defect model in the role of recombinant adenovirus vector. Methods hBMP7 cDNA cloned into the shuttle plasmid pACCMV plpA, build pACCMV/rhBMP7 shuttle plasmid, Application of calcium phosphate DNA coprecipitation method PJM17 shuttle plasmid and packaging plasmids were transfected into 293 cells, the intracellular homologous recombination to construct bone morphogenetic protein-7 recombinant Ad pACCMV/rhBMP7. was successfully constructed the recombinant plasmid pACCMV / rhBMP7, by 293 cells, the amplification of purified virus titer was 1 1011pfu/mL. Conclusion The construction of human bone morphogenetic protein-7 recombinant Ad pACCMV/rhBMP7, the bone defect, nonunion of gene therapy basis. [Keywords:] bone morphogenetic protein-7, adenovirus vector, gene transfer, titer ABSTRACT: Objective In order to investigate the treatment of bone defect repairing by recombinant adenovirus transudation the hBMP7 gene, the vector of hPMP7 recombinant Ad was constructed. Methods The gene of hBMP7 was inserted into the replicated defective adenovirus shuttle plasmid pACCMV plpA. We constructed the vector of hBMP7 recombinant Ad. The recombinant Ad viral stock was packaged. Totally 293 cells were co transfected with the rAd vector of plasmid pACCMV/rhBMP7 and packaging plasmid PJM17 by calcium phosphate precipitation. Results The recombinant viral vector of plasmid pACCMV/rhBMP7 was constructed successfully. We got the Ad pACCMV/rhBMP7 stocks of high tire 1 1011 pfu / mL. Conclusion We prepared the viral stock of rAd BMP7 that can serve as the experimental study of gene therapy of bone defect repairing. KEY WORDS: bone morphogenetic protein; recombinant adenovirus; gene transfer; titration With the m