Human interleukin-24 gene induces apoptosis in human cervical cancer Ca Ski Experimental Study.docVIP

 PAGE \* MERGEFORMAT 7 Human interleukin-24 gene induces apoptosis in human cervical cancer Ca Ski Experimental Study [Keywords:] human interleukin-24; Ca, Ski cell; papilloma virus, human;, E6 gene; protein P53; cell apoptosis Apoptosis induction of human cervical cancer Ca Ski cells by hIL24 gene [Abstract] AIM: To investigate the hIL24 gene inducing apoptosis of human cervical cancer Ca Ski cells and its possible mechanism. METHODS: Ca Ski cells were transfected with hIL24 eukaryotic expression vector pcDNA3.1 () hIL24 by lipofectamine2000. The effect of hIL24 on the transcription of HPV E6 gene was analyzed by RTPCR. The effect of hIL24 on P53 protein was analyzed by Western Blot. Apoptosis of Ca Ski cells induced by hIL24 gene was analyzed by FACS. RESULTS: Transcription level of HPV E6 gene decreased significantly, the amount of P53 protein increased significantly and the apoptosis rate of Ca Ski cells increased significantly after hIL24 transfection. CONCLUSION: HIL24 gene can inhibit the transcription of HPV E6 gene, recover the activity of P53 protein, then induce the apoptosis of Ca Ski cells. [Keywords] human interleukin24; Ca Ski cells; papillomavirus, human; E6 genes; protein P53; apoptosis [Abstract] Objective: To study the human interleukin-24 (hIL24) in vitro induced apoptosis of cervical cancer Ca Ski cells and its mechanism. Methods: ① to liposome recombinant eukaryotic expression plasmid pcDNA3.1 () hIL24 transfection Ca Ski cells; ② using RTPCR detection of Ca Ski cells after treatment HPV E6 oncogene mRNA level changes; ③ Western Blot analysis of the treated Ca Ski cell changes in the level of tumor suppressor protein P53; ④ analyzed by flow cytometry after treatment The Ca Ski cell apoptosis. Results: Ca Ski cells after treatment HPV E6 oncogene mRNA levels decline in the level of tumor suppressor protein P53 increase in apoptosis rate increased. Conclusion: The eukaryotic expression vector-mediated gene hIL24 In vitro transfectio