Hypericum perforatum extraction process of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 12 Hypericum perforatum extraction process of total flavonoids [Abstract] Objective of Hypericum perforatum extraction process of total flavonoids were optimized. Methods orthogonal test, as an indicator of total flavonoids, on ethanol concentration, solvent volume, extraction time and extraction times of 4 factors were . The results will be used 8 times Hypericum extract 70% ethanol 3 times, 2 h, to obtain a higher extraction rate. Conclusion The process of extracting effective components higher, repeatable, stable and feasible. [Keywords:] Hypericum, flavonoids, hyperin, extraction Abstract: Objective To optimize the extraction process of total flavonoids in Hypericum perforatum L.. Methods The concentration and volume of alcohol, extraction time and frequency were systematically studied by the total flavonoids as the marker. Results The more effective way to extract total flavones was extraction with 8 times amount of 70% alcohol under reflux for 3 times and 2 h for each time. Conclusion This method obtains more effective components. It is stable and reliable. Keywords:: Hypericum perforatum L., total flavonoids, hyperoside, extraction process Hypericum perforatum for the 2005 Edition lt;lt;Chinese Pharmacopoeia “(a) the new varieties of herbs, is Section Garcinia Hypericum Hypericum Hypericum perforatum L. aerial part dry, with comfortable liver Jieyu, heat and dampness, swelling and pain and other effects, for the situation go rather smooth, depressed qi, joint swelling, pain, difficult urination and so on. Some studies show that flavonoids in Hypericum perforatum has hepatoprotective, anti-hepatitis B virus and choleretic effect, and one of the best hyperin. We in the past on the basis of Hypericum perforatum extraction process of total flavonoids further research, are reported below. An instrument and reagent Hypericum perforatum Hypericum perforatum L. were purchased from the Kowloon side of Bozhou medicine co