In Chinese Han population HLA class classical haplotype and linkage analysis.docVIP

 PAGE \* MERGEFORMAT 8 In Chinese Han population HLA class classical haplotype and linkage analysis Authors: Yuan Fang, Yu-Ying Sun, Luo Yuan, Liang Fei, Liu Nan, Jinli, LIU Jin-feng, Liu Shuguang, XI Yongzhi Abstract This study aimed to explore the Chinese Han population HLA Ⅰ class classical loci HLA  A, HLA  B, HLA  Cw locus allele frequencies, haplotype frequencies and linkage characteristics. Using sequence-specific primers (SSP) PCR low-resolution genotyping of 1 014 cases of randomly selected individual unrelated to the classical HLA Ⅰ type locus genotyping, and its associated genetic parameters were analyzed statistically. The results showed that the Han population in the more common HLA  Ⅰ genes are A * 02 (0.33), A * 11 (0.24), B * 15 (0.14), B * 13 (0.13), Cw * 03 (0.25), Cw * 07 (0.18); the more common haplotype was A * 02  B * 46 (0.071), A * 11  B * 15 (0.051), A * 02  Cw * 01 (0.084), A * 11  Cw * 03 (0.079), B * 46  Cw * 01 (0.095), and B * 13  Cw * 03 (0.071) and so on, in which A * 02  B * 46, A * 30  B * 13, A * 30  Cw * 06, A * 02  Cw * 01, B * 46  Cw * 01 and B * 58  Cw * 03 and other haplotypes showing significant linkage disequilibrium; and A * 02  B * 15 , A * 02  B * 40, A * 24  Cw * 03, A * 02  Cw * 03, A * 31  Cw * 03 and other relatively low linkage disequilibrium, in which A * 24  Cw * 03 in the recombination events in more often. In addition, A * 02  B * 46  Cw * 01 (0.075), A * 30  B * 13  Cw * 06 (0.046), A * 11  B * 13  Cw * 03 (0.045), A * 33  B * 58  Cw * 03 (0.044), A * 11  B * 15  Cw * 08 (0.027), A * 02  B * 38  Cw * 07 (0.023), A * 11  B * 40  Cw * 07 (0.022) such as the common extended haplotype. Conclusion: This study systematically analyzed the HLA Genetics in Chinese Han population distribution characteristics of evolution for the group, clinical transplantation and disease-related research provides a valuable genetic data. 【Keywords】 genetic polymorphism