In situ rat kidney transplant model of technological improvement.docVIP

 PAGE \* MERGEFORMAT 25 In situ rat kidney transplant model of technological improvement 【Abstract】 Objective: To enhance the rat model of renal transplantation of donor kidney utilization of right donor nephrectomy, rats improved method of ureteral anastomosis to establish were cut from the same donor kidneys with bilateral kidney transplants were carried out in situ method of . Methods: Donor harvesting the left and right kidneys, renal arteries through the perfusion; donor kidney renal artery and vein were the recipient left renal artery and vein vascular anastomosis and the application of a temporary stent-assisted method for the kidney ureter and recipient ureteral anastomosis, and removal of receptors on the right kidney. Comparing the left and right kidney receptor for the survival and postoperative complications, and slice through the pathological evaluation of histological changes of the graft. Results: From 30 donor rats were cut 52 kidneys, respectively, the purposes of the left and right kidney for kidney transplant patients 28,24. Compared with conventional unilateral kidney in rats to save 42%. 5d for the group of animals, the left kidney survival rate of 89.3% (25/28), the right kidney group 5d animal survival rate 91.6% (22/24), using left and right side of the receptor for the survival of renal graft histology changes had no significant effect. Conclusion: This method can save experimental time, reduce the number of donors, in line with animal experiments in the economy and ethics requirements. Keywords: Kidney shifting microsurgical technique for nephrectomy in rats Rat kidney transplant model is to conduct an important tool in transplantation immunology research, but also to micro-surgery training, classical model, is widely used in acute renal transplantation, chronic rejection, renal hypertension, micro-surgery technology-related experimental study . Lee [1] in 1965, making this model was first reported by the specific