Induction of autotetraploid Artemisia annua L..docVIP

 PAGE \* MERGEFORMAT 8 Induction of autotetraploid Artemisia annua L. Author: Wu Xiaoli Liu Fei, Zhong-Yue Li Longyun [Abstract] Objective To induce tetraploid Artemisia annua, filtering out the best conditions for mutagenesis. Methods of materials mutation in two ways. Seedling immersion method: Colchicine seedlings soaked in Artemisia annua, liquid concentration and soaking time and processing temperature gradient settings; germinating seed soaking method: Colchicine germinating seeds soaked in Artemisia annua, liquid concentration gradient set up. Then derived from seedling root tip chromosomes microscopy, combined with plant shape and characteristics of stomatal guard cells to determine its mutation rate. Results seedling immersion method in order to 15 ℃ , the 0.2% colchicine 96 h is appropriate, mutation rate of 30%. Germinating seeds soaking in, to 25 ℃ , 3 ~ 5 mg / L colchicine 120 h is appropriate, mutation rate of 45% ~ 96%. Conclusion is better than seedlings germinating seeds soaking soaking. [Keywords:] tetraploid colchicine chromosome Artemisia annua Artemisia annua L. , Also known as Artemisia annua, a medicinal herb annual Asteraceae. Its extract artemisinin malaria and cerebral malaria chloroquine antimalarial properties of a highly efficient and low toxicity, WHO has identified compound artemisinin-based medicines for the most safe and effective antimalarial drugs. Now the world has 51 countries and regions in the WHO recommendation be designated as anti-malarial drugs, which makes the global demand for artemisinin-based raw materials soared, beginning in 2004 have emerged from the raw materials to finished all aspects of drug serious supply shortages. Artemisia annua on the international market price of raw materials rose from 240 U.S. dollars per ton to 700 U.S. dollars [1]. Although at present the drug can be synthesized, but the cost is very high, not industrial [2], industrial plants, mainly from Artemisia annua L. extract. For long-t