Inflammatory and immune deficiency and blood stasis in rats on the analysis of differential gene expression.docVIP

 PAGE \* MERGEFORMAT 11 Inflammatory and immune deficiency and blood stasis in rats on the analysis of differential gene expression Of: Liu Ya Li Xiaohui Zhang harbor weeks she thinks Xianzhijiayi [Abstract]: Objective To study the inflammatory immune deficiency blood stasis of molecular mechanism. Methods 12 rats were randomly divided into qi and blood stasis model group and normal control group. Application of the whole gene chip on Qi and Blood wide rats and normal rats gene expression detection, analysis, and differential expression of inflammatory and immune-related genes. Results deficiency and blood stasis model group than in normal rats and upregulated 831 genes, 563 known genes, 782 genes downregulated have been 544 known genes were identified. in the known genes, the inflammatory immune response associated with upregulation of 50 genes, 17 genes downregulated. Conclusion inflammatory and immune-related genes revealed differential expression and abnormal Qi Deficiency closely related to the inflammatory immune response. [Keywords: animal model with Qi inflammatory and immune response gene chip Abstract: Objective To investigate molecular biological basis associated with inflammatory and immune response in Qi deficency and blood stasis syndrome. Methods Twelve rats were randomly divided into model group and control group. Rat Genome 230 2.0 Array Gene Chip was used for examination of gene expression profiles in rats with Qi deficiency and blood stasis syndrome and normal rats. Differental expression genes associated with inflammatory and immune mechanism were analyzed. Results Compared with the control group, there were different expression genes in model group, the up-regulated were 831 genes, known functional genes 563, and down-regulated 782, known functional genes 544. The expression level increased in 50 genes and decreased in 17 genes associated with inflammatory and immune response. Conclusion Differental expression genes associated w