Medicine cleared hydroxyl radical improvement of the experiment.docVIP

 PAGE \* MERGEFORMAT 8 Medicine cleared hydroxyl radical improvement of the experiment Authors: Yuqing Gao, Liu Jie frequency, Wu Mei-Qing [Abstract] Objective To phenanthroline-Fe2 oxidation method modified to make it in time for the free radical scavenger screening more effective and more sensitive. Methods in different pH environments, different holding time and different wavelengths under the conditions of the determination of comparative experiment. Results Phenanthroline-Fe2 complex in the pH 4.5 environment, and the determination of 510 nm wavelengths greater absorbance, 30 min of holding time to enable the possession of a larger and more stable absorbance. Conclusion of the phenanthroline - Fe2 oxidation experiments improved the method can increase the sensitivity of screening free radical scavengers. [Keywords:] free radical scavenger; o-phenanthroline; hydroxyl radical Abstract: ObjectiveThe improvement can be carried out to the oxidation of phenathroline-Fe2 in order to be effective and more sensitive in screening the free radical scavenger.MethodsMaking experiments by comparing the conditions of different pH, heat preservations and various wavelengh.ResultsThe complex of phenathroline -Fe2 had great absorbance with 510 nm wavelength in the environment of pH4.5, and the absorbance was larger and more stable after the 30 min heat preservation.ConclusionThe improvement can increase the sensibility for sereening the free radical scavenger. Keywords:: Free radical scavenger; Phenathroline; Hydroxy radical The body in a variety of free radicals produced during metabolism, including superoxide anion radical (O-2 * ), hydroxyl radical (* OH) and its active derivatives, such as H2O2, of which the OH chemical properties of the most lively. A certain amount of oxygen free radicals can be used for the body, but too much will be the role of oxygen free radicals in the body proteins, nucleic acids, lipids and other biological molecules, causing damage to c