Mice model of chronic inhalation of n-hexane to establish.docVIP

 PAGE \* MERGEFORMAT 5 Mice model of chronic inhalation of n-hexane to establish Author: Da-Yong Zhang, Huang Xin, Cao Jing Ting, Xin Cheng, Chen Ying [Abstract] Objective To establish the chronic inhalation of n-hexane animal experimental model to study the long-term inhalation of n-hexane toxicological injury mechanism of experimental methods. Methods 24 Kunming mice were randomly divided into four groups (normal control group, exposed to 4,8 and 12 weeks group), the static type exposure, the initial concentration of 17.6g/m3. After exposure, blood samples measured the concentration of n-hexane; the lung, liver and kidney of conventional fixed, embedded, light microscopic observation of structural changes. Results The blood of mice exposed groups were significantly higher than average concentration of n-hexane and normal control group, has reached the required exposure. Microscopic observation of mice lungs, liver and kidneys suffered from pathological changes, and with prolonged exposure showed increased pathological changes in the trend. Conclusion In this study, n-hexane used in Kunming mice were exposed to static-type methods, has resulted in mice carried the major metabolic organ injury, can be used as toxicological study of injury mechanism of n-hexane reference model. [Keywords:] n-hexane N-hexane (n  hexane) is a saturated aliphatic hydrocarbons, is a commonly used organic industrial solvents. Their prices are low, in the printing, paint, electronic equipment cleaning industry has been widely used. In recent years, long-term exposure caused by chronic n-hexane poisoning frequently occur, and there is increasing trend 〔 1,2〕 . N-hexane on the body injury, is a long process after exposure to chronic poisoning. The animals exposed to the previous experimental model of n-hexane, n-hexane is mainly acute injury model, and the specifications and methods are not unified, not a very good observation of n-hexane, after chronic inhalation of its main meta