Mice of different ages in the glutathione system of brain tissue compared.doc

 PAGE \* MERGEFORMAT 12 Mice of different ages in the glutathione system of brain tissue compared Of: Liangqing Ning Xia Yu-Min Yang Ye Sheng Yuchen Lily Chiang Ping quarter [Abstract] Objective To investigate the brain tissue of mice of different ages of glutathione (GSH) levels and related enzyme activity differences. Comparison of 3,8 and 24 week old mouse brain tissue GSH content and GSH synthetase (GCL), GSH reductase (GR), GSH peroxidase (GSH Px) and GSH sulfhydryl transferase (GST) activity. The results of mice of different ages in brain GSH content, GCL, and no significant changes in GR activity and 24 weeks .8 Brain Age was significantly lower than the GSH Px weeks 3 weeks of age group .24 GST activity was significantly higher than that of mouse brain 3 and 8 weeks of age. Conclusion Mouse Brain GSH content and synthesis of GCL and GR activity does not vary with age of significant change, while the GSH dependent antioxidant enzyme activity of GST and GSH Px is a significant difference. [Keywords:] glutathione, glutathione synthetase, glutathione reductase, glutathione peroxidase, glutathione transferase thiol [Abstract] Objective To investigate the difference of glutathione contents and the activities of glutathione related enzymes in mice brains of different age.Methods The mice were divided into 3 groups: 3,8 and 24 weeks group respectively, and 10 mice in each group. The glutathione contents, and the activities of glutamate cysteine ligase (GCL), glutathione reductase (GR), glutathione peroxidase (GSH Px), glutathione S transferase (GST) were observed in mice brains of different age.Results There were no significant differences in glutathione contents, GCL and GR activities in brains between different age mice.However, the GSH Px activity was lower in 8 and 24 weeks group than that in 3 weeks group.The GST activity in 24 weeks group was higher than that in 3 and 8 weeks groups.Conclusions The glutathione contents and the activities of GC