Micro-gel technology in neonatal hemolytic disease detection.doc

 PAGE \* MERGEFORMAT 7 Micro-gel technology in neonatal hemolytic disease detection [Keywords:] microcolumn gel test serum hemolytic disease of newborn Generally refers specifically to maternal and child neonatal hemolytic disease caused by fetal blood group incompatibility or neonatal immune hemolytic disease (haemolytic disease of the newborn, referred to as HDN. In China, due to ABO blood group incompatibility hemolytic disease of newborns caused by more common, Rh ABO hemolytic disease of newborns caused by the second. severe hemolytic disease of newborn, kernicterus can occur, leading to mental hypoplasia, cerebral palsy and other complications. hemolytic disease of newborns is a serious disease of newborn health. condensate using micro-column plastic technology HDN three diagnostic tests (direct antiglobulin test, antibody elution test, serum free antibody test) the rapid detection, more timely and beneficial to the clinical diagnosis and treatment of children. microcolumn gel Technology (microcolumn get Immunoassay at home and abroad serological screening method. Its advanced design theory, is simple rapid, specimens with less, the results easy to determine, with high sensitivity and specificity advantages. 1 Materials and methods 1.1 Reagent and instrument reagents: DianaGeL coombs cards (each card contains eight micro-column tube holes, each hole tube micro-column buffer with low ionic strength (LISS particles immersed in a sieve, with polyvalent anti-human globulin (rabbit IG polyclonal and monoclonal anti-C3d anti-cloning 12011.D10. Standard RBC: 0.8% O-type screening cells (Lot: 8004801 801001 801101 801201 900201 900101 900301 900401.0.8% A 0.8% B-type cell-type cell batch number: 80018018001901 80078018002501 900201 901 001 900 501 900 601 901 001. Diasnvstic Grifols SA card: for ABO, Rh blood group, direct antiglobulin test, batch number: 5,004,010,160,010,101 8,001,020,180,010,301 8,003,010,180,060,201. D