Microbial secondary metabolite biosynthesis gene cluster and Drug Innovation.docVIP

 PAGE \* MERGEFORMAT 32 Microbial secondary metabolite biosynthesis gene cluster and Drug Innovation Keywords:, microbial secondary metabolites;,, biosynthesis gene cluster;,, drug innovation;,, combinatorial biosynthesis;,, metabolic engineering Abstract: The micro-organisms produce a number of structure and biological activity of a variety of secondary metabolites, their biosynthesis gene cluster of cloning is the increase in production of drug innovation and a necessary prerequisite. To date, more than 150 bio-synthetic gene cluster in various ways been cloned and used to combinatorial biosynthesis, in vitro sugar randomized, metabolic engineering orientation transformation. Our laboratory has been cloned and determined aminoglycoside Jinggangmycin / validamycin, multi-vinyl antibiotics FR008 / Candicidin, polyether Nanchangmycin, poly ketones meilingmycin, hybrid polyketide more ADM, etc. I oxazole peptide biosynthesis gene cluster. In-depth analysis of gene function has revealed their unique biosynthetic pathway and regulation mechanism for the ongoing structural transformation of combinatorial biosynthesis and metabolic engineering laid the foundation for increase in production. Keywords:: microbial secondary metabolites; biosynthetic gene cluster; drug innovation; combinatorial biosynthesis; metabolic engineering Secondary metabolic pathway genes and new drug discovery ABSTRACT Microorganisms produce myriads of secondary metabolites with both structural and functional diversities. The cloning of corresponding biosynthetic gene clusters is essential for new drug discovery and yield improvement by metabolic engineering. To date, more than 150 biosynthetic gene clusters had been cloned via different strategies, which are subsequently manipulated through combinatorial biosynthesis, in vitro glycorandomization, or other biotechnological methods. In our laboratory, several biosynthetic gene clusters have been cloned and sequenced