MRSA-PBP2a monoclonal antibody latex agglutination assay for the establishment of.docVIP

 PAGE \* MERGEFORMAT 17 MRSA-PBP2a monoclonal antibody latex agglutination assay for the establishment of [Abstract] Application of anti-PBP2a monoclonal antibodies, by chemical crosslinking of carboxylated polystyrene latex allergens, a rapid detection of MRSA-PBP2a latex agglutination. [Method] to bitterness - ammonium sulphate purified PBP2a monoclonal antibody, monoclonal antibodies and the purified latex carboxylation by carbodiimide (EDC) covalent coupling, and coupling conditions (coupled antibody concentration, latex concentration , coupling time and the coupling buffer) to explore and optimize the establishment of PBP2a latex agglutination test. [Results] 140 mg / L of antibody concentration and 15 g / L of carboxyl polystyrene latex, pH 5.8 buffer solution at 8 h after conjugated good coagulation effect and coupling efficiency, the establishment of The sensitivity and specificity of latex agglutination good. [Conclusion] initially established to detect PBP2a latex agglutination assay for rapid identification of MRSA kit development has laid a good foundation. [Keywords:] Methicillin-resistant Staphylococcus aureus Methicillin-resistant Staphylococcus aureus (methicillin resistant staphylococcus aureus, MRSA) is a global cause of nosocomial infection is one of the most important drug-resistant pathogens [1,2], commonly used, including methicillin, including a variety of extensively drug-resistant antibiotics, the main resistance mechanism is due to chromosomal mecA gene [3] codes generated pairs of β -lactam antibiotics with low affinity penicillin binding protein 2a (penicillin binding protein 2a, PBP2a) caused by, because of its due infected with a complex condition, treatment difficult, high mortality, the need for rapid and accurate method of diagnosis of MRSA infection in a country and research scholars are concerned about hot spots. Nakatomi and Sugiyama [4] in 1998 reported that a rapid detection of MRSA latex agglutination test (Latex agglut