Musca domestica sequence-related amplified polymorphism amplification system Establishment and Optimization of.docVIP

 PAGE \* MERGEFORMAT 13 Musca domestica sequence-related amplified polymorphism amplification system Establishment and Optimization of Author: Liu Hong Mei, Fang Xiaobo, ZHENG Qin Ni, Hu Shiming, Jian-Wei Wu [Abstract] Objective: To establish and optimize the housefly sequence-related amplified polymorphism (SRAP-PCR) amplification system. Methods: The domestication and houseflies as material, through a single factor experiment studied the template DNA, Mg2, dNTPs, primer concentration and the amount of Taq DNA polymerase housefly SRAP molecular marker system and the impact of amplification, and amplification system was optimization. Results: The optimized total volume of reaction system 30 μ l, containing 50 ng template DNA, 1.5 mmol / L Mg2, 0. 20 mmol / L dNTPs, 0. 15 μ mol / L primer and 0. 50 U Taq DNA polymerase. Conclusion: The house fly SRAP-PCR amplification of the establishment of optimized system for future use of genetic diversity of Musca domestica SRAP technology analysis, laid the foundation for gene mapping. [Keywords:] Muscidae; random amplified polymorphic DNA technique; genetic marker [Abstract] Objective: To establish and optimize SRAP-PCR reaction system of housefly. Methods: Effects of concentrations of template DNA, Mg2, dNTPs, primers and amount of Taq DNA polymerase on SRAP molecular marker amplification system of Musca domestica L were studied in monofactorial experiment, and the amplification system was optimized. Results: Total volume of the optimal system was 30μ l containing 50 ng of template DNA, 1.5mmol / L of Mg2, 0. 20 mmol / L of dNTPs, 0. 15μ mol / L of primers and 0. 50 U of Taq DNA polymerase. Conclusion: This system provides a useful tool for analysis of genetic diversity and gene location of Musca domestica L. [Keywords:] Muscidae; random amplified polymorphic DNA technique; genetic markers Housefly (Musca domestica L) is an important medical vector insects can spread dysentery, typhoid, cholera, polio and other dis