Muscle flap wrapped in tracheal autograft complexes BMP-induced cartilage regeneration.doc

 PAGE \* MERGEFORMAT 3 Muscle flap wrapped in tracheal autograft complexes BMP-induced cartilage regeneration Author: Lu Qiang, Xiao-Fei Li, Cheng QS, Wang Xiaoping, Han Yong, Wang law, Jian Wang, Yan Xiaolong [Keywords:] Trachea / transplantation BMPinduced cartilage regeneration in tracheal autograft with muscular wrapping [Abstract] AIM: To study the regeneration of cartilage by rhBMP2 in tracheal autograft with muscular wrapping. METHODS: Fifteen mongrel dogs were randomly and equally divided into 3 autotransplanted groups: group wrapped with muscle (Group A), group wrapped with muscle and implanted with rhBMP2 (Group B) and group with no treatment (Group C). A fivering cervical tracheal segment was harvested as the autograft. The animals were sacrificed at 1, 2, 4, 8 and 10 weeks postoperative. The general conditions of animals, gross and histological character of the specimens and microvessel density were examined and modified CaCo method was used to detect alkaline phosphatase (ALP). RESULTS: Little regeneration of cartilage was found in Group C while obvious regeneration of cartilage was found in Group A and Group B . Significant difference was found in ALP within 8 weeks after operation between the three groups, with Group B the highest, followed by Group A and then Group C, while no significant difference was found in ALP 8 weeks after operation between the 3 groups. CONCLUSION : Local use of bone morphogenetic protein (BMP) may upregulate ALP in tracheal cartilage and inhibit the absorption of tracheal cartilage. Muscle wrapping can increase the microvessel density and RhBMP2 helps to maintain tracheals structure. [Keywords] trachea / transplantation; alkaline phosphatase; bone morphogenetic protein2; muscular wrapping [Abstract] Objective: To study the BMP composite muscle flap in autologous transplantation of cartilage regeneration. Methods: 15 dogs were randomly divided into three groups, all five rings were cut cervical tracheal segment a