Mutant human APP gene and enhanced green fluorescent protein fusion vector.doc

 PAGE \* MERGEFORMAT 11 Mutant human APP gene and enhanced green fluorescent protein fusion vector Of: Zhang Lijuan Cheng Min Liu Rong Wang Fengbin [Abstract] Objective To construct carrying mutant human amyloid precursor protein gene (APP695) and enhanced green fluorescent protein (EGFP gene fusion vector. Methods NCBI locate APP695 sequence primers. The pCB6 plasmid carrying the wild-type APP695 sequence as template, primers mutant mutant APP695. the mutant APP695 with the pIRES2 EGFP connection and sequencing. Results After restriction enzyme digestion, PCR and DNA sequencing methods, confirmed that construction of mutant plasmid pIRES2 EGFP/APP695 correct sequence. Conclusion The construction of APP695 EGFP fusion gene vector for the study of the molecular mechanism of AD pathogenesis and treatment of drug screening when the foundation. [Keywords:] Swedish mutant APP695, enhanced green fluorescent protein, APP695 EGFP fusion gene vector [Abstract] Objective To construct and express a recombinant vector bearing fusion gene of human amyloid precursor protein (APP) 695 mutant gene and enhanced fluorescence protein (EGFP) fusion gene. Methods The primers were designed according to the sequences of APP695 gene found from NCBI . Then the mutant APP695 was amplifed by PCR with pCB6 carrying wild human APP695 and acting as a template, the gene of mutant APP695 was ligated to pIRES2 EGFP. The recombinant plasmid of pIRES2 EGFP/APP695 mutant was verfied by checking sequences. Results The exact sequences of pIRES2 EGFP/APP695 mutant vector were confirmed by digestion of restriction endonucleases, PCR and sequencing. Conclusions The pIRES2 EGFPAPP695 mutant fusion gene recombination has been constructed successfully, which lays the foundation for further research of AD pathogenesis and screening new drug targets on AD therapy. [Keywords:] Swedish mutant APP695, EGFP, Fusion gene of human APP695 / EGFP; Vector Amyloid precursor protein (amyloid precurs