Nasopharyngeal Carcinoma Screening of differentially expressed miRNAs.doc

 PAGE \* MERGEFORMAT 26 Nasopharyngeal Carcinoma Screening of differentially expressed miRNAs Authors: Li Xiaoxia, Li Ruiping, Du Zi-ming, Zeng Mu-sheng, Shao Jianyong 【Abstract】 【Objective】 application miRNAs chip screening of nasopharyngeal carcinoma cells in primary culture of differentially expressed miRNAs, and to find differentially expressed miRNAs regulation of target genes for further study of its role in the pathogenesis of nasopharyngeal carcinoma basis. 【Methods】 miRNAs microarray screening of nasopharyngeal carcinoma cells in primary culture of differentially expressed miRNAs, while the use of primers specific miRNAs differentially expressed miRNAs group fluorescence quantitative RT PCR validation. The use of target gene prediction software in combination with whole-genome expression microarray results to predict differential expression of miRNAs may be regulated target genes, and the use of western blot detection of predicted target genes in primary cultured nasopharyngeal carcinoma cells. 【Results】 miRNAs chip results showed that nasopharyngeal miR-203, miR-503, miR-424, miR-141 and miR-148a expression decreased and the miR-25, miR-195 and miR-15a expression increases, its differential expression were two-fold or more; fluorescence quantitative RT PCR results and the results more in line with miRNAs chips; of these miR-203 predicted target genes for CCNG1 and SPARC, Western blot results showed that in primary cultured nasopharyngeal carcinoma cells are also high - expression. 【Conclusion】 miR-203, miR-503, miR-424, miR-141, miR-148a, miR-25, miR-195, miR-15a in the primary cultured nasopharyngeal carcinoma cells and normal nasopharyngeal epithelial cells present in differential expression; CCNG1 and SPARC may be regulation of miR-203 target genes may be involved in the occurrence and development of NPC. Keywords: microRNA; nasopharyngeal carcinoma; differential expression Abstract: 【Objective】 To investigate the diffe