Nimodipine in rats in vivo pharmacokinetic studies.doc

 PAGE \* MERGEFORMAT 10 Nimodipine in rats in vivo pharmacokinetic studies Author: Liu Xiaolan Chen Yu-ying Miss Rachel Lau [Abstract] Objective: To investigate the rat blood plasma concentration of nimodipine in the determination of its pharmacokinetics, the establishment of HPLC determination of nimodipine in rat plasma by HPLC method. Methods: The pharmacodynamics - pharmacokinetics unified model, as well as the HPLC determination. Results: The column was Hyperil C18 column (4.6 * 200mm, 5μ m), mobile phase was methanol - water (87 ︰ 13), flow rate 1.0ml/min, column temperature 35 ℃ , detection wavelength 240nm. Nimodipine plasma in the 10 ~ 3200ng * ml-1 range, with good linear relationship, Y = 691C +415, R = 0.9998, extraction recovery of 90.4% ~ 103.0%, recovery was 97.0% ~ 105.3%. Rat gavage 10mg * kg-1, 20mg * kg-1, 40 mg * kg-1. Plasma concentration time curves of absorption consistent with a two-compartment model, the dose and AUC, Tmax has a good linear relationship. Conclusion: Nimodipine disposition in rats belonging to the linear dynamics, pharmacokinetic parameters and dose-related. Law stable, simple, reliable and can be used for nimodipine plasma concentration analysis and pharmacokinetics. [Keywords:] high-performance liquid chromatography of blood concentration of nimodipine pharmacokinetics Nimodipine is a clinical treatment of cerebral vascular disease on the widely used calcium channel blocker. This study investigated the use of high-performance liquid chromatography determination of nimodipine in rat blood plasma concentration, the establishment of HPLC determination of nimodipine in rat plasma by HPLC method and its pharmacokinetics. 1 Materials and methods 1.1 drugs, animal drugs and reagents and instrumentation: production of a pharmaceutical factory nimodipine, nimodipine reference substance (Chinese medicines and biological products), pure methanol, chromatography and other reagents of analytical pure. Animals: rats, we