Nimesulide force in human gastric cancer orthotopic transplantation model in rats.doc

 PAGE \* MERGEFORMAT 10 Nimesulide force in human gastric cancer orthotopic transplantation model in rats [Keywords:] gastric cancer [Abstract] Objective To investigate the effect of cyclooxygenase inhibitor on tumor metastasis, tumor angiogenesis and vascular endothelial growth factor (VEGF of orthotopic implanted human gastric carcinoma in non-obesity diabetes (NOD severe combined immune deficiency (SCID mice. Methods Human gastric cancer SGC -7901 were orthotopically implanted into the stomach of the NOD SCID mice. Forty mice were randomly divided into two groups which received either intravenous injections of 0.9% NaCl solution (0.9% NaCl solution group of 50mg / (kg d Nimesulide (Nimesulide group twice weekly for two weeks. Mice were sacrificed five weeks after implantation. Tissues from stomach and other organs were obtained for histopathological evaluation. The intratumoral microvessel density (MVD and VEGF protein expression in tumor were evaluated immunohistochemically. Results The tumor metastasis rates were 18/20 in 0.9% NaCl solution group and 5 / 20 in Nimesulide group (P lt;0.05. MVD was 9.8 + -3.9 in 0.9% NaCl solution group and 4.4 + -2.4 in Nimesulide group (P lt;0.05. VEGF protein expression was 90% in 0.9% NaCl solution group and 25% in Nimesuli group (P lt;0.05. Conclusion Nimesuli can inhibit the metastasis or gastric cancer through inhibiting tumor VEGF expression, and tumor angiogenesis with no obvious anticoagulant activity. [Keywords:] Nimesulide; stomach neoplasms; neoplasm metastasis; angiogenesis; vascular endothelial growth factor Tumor metastasis is a key factor to death [1]. Has been found, vascular endothelial growth factor (VEGF can promote tumor angiogenesis. Inhibiting VEGF can inhibit tumor angiogenesis and tumor metastasis [2]. In vitro experiments showed that cyclooxygenase inhibition force agent nimesulide can inhibit VEGF, thereby inhibiting endothelial cell proliferation and angiogenesis. In this study, gast