NO donor isoflavone Synthesis and biological activity.doc

 PAGE \* MERGEFORMAT 13 NO donor isoflavone Synthesis and biological activity Of: Lifang Yao, Yang Xinping, Lin Xun, Lao Shi Xin [Abstract] Objective To design and synthesize a new type of NO releasing isoflavone derivatives and the isoflavones to the adoption of the synergistic effect of NO, enhanced anti-tumor activity of derivatives. Methods In this paper, the carbon chain of organic nitrates and isoflavone 7 - phenol hydroxy connection, get two 7 - substituted derivatives of isoflavone nitrate. The results were characterized by IR, 1H-NMR, 13C-NMR and MS characterization, the total yield was 40% to 45%. Conclusions of the target product was Determination of nitric oxide release in vitro and in vitro antitumor activity test results show that the products released within 2h of nitric oxide in the isosorbide mononitrate is 3 times, all of which have some antitumor activity. [Keywords:] isoflavones; NO donor; synthesis; anti-tumor activity Isoflavones are present in the beans in a wide range of plant estrogen, is the active ingredient in a variety of herbs, can adjust the body’s endocrine, prevention and treatment of women with menopausal symptoms [1], reduce blood fat and reduce the incidence of coronary heart disease, prevention of bone quality of osteoporosis, cancer and relieve pain and other active [2]. studies have shown that genistein, genistein and daidzein and chickpea isoflavones and other natural flavin A good anti-tumor activity, and is a kinds of powerful protein tyrosine kinase (PTK) inhibitor, can inhibit DNA topoisomerase Ⅱ activity and inhibition of tumor cell DNA synthesis, inhibition of angiogenesis [3]. NO is the body of important cellular messenger molecules and effector molecules , involved in regulating a variety of physiological functions, have important physiological and pharmacological effects, if the body may lead to NO deficiency hypertension, atherosclerosis, coronary heart disease, acute myocardial infarction, lung and