p53 and vascular endothelial growth endostatin gene transfection of HL60 cell growth on.docVIP

 PAGE \* MERGEFORMAT 6 p53 and vascular endothelial growth endostatin gene transfection of HL60 cell growth on [Abstract] Objective To observe the p53 and vascular endothelial growth endostatin gene (AS) were transfected HL60 cell growth and vascular endothelial growth factor (VEGF), bax, bcl  2 expression level, and to explore jointly the possibility of gene therapy of acute leukemia sex. Methods liposome p53 AS transfected HL60 cells to RT  PCR assay of transfected cells in gene expression. Through cell colony formation assay, MTT test and flow cytometry after transfection, p53 AS on HL60 cell growth. Using RT  PCR and immunohistochemical detection of p53 AS transfected HL60 cells, VEGF, bcl  2 and bax expression changes. Results p53 AS transfected successfully and stably expressed in HL60 cells, significantly inhibited cell growth, coordinated the promotion of apoptosis, the cell’s expression of VEGF and bcl  2 decreased, bax expression was elevated. Conclusion p53 AS transfection on the HL60 cells significantly inhibit the growth of the two there is synergy, which may be affecting the bcl  2/bax. [Keywords:] gene; p53; angiostatin category; leukemia; HL60 cells; apoptosis Gene therapy is a cancer treatment in recent years, hot, but the effect is not ideal. One reason is that the incidence of tumor development is a complex process, involving multiple genetic abnormalities and various other factors, the role of a single gene therapy can only act on the tumor occurrence and development of a certain part of the process, only the relevant genes are working together in order to more effectively inhibit tumor. p53 gene is an important tumor suppressor gene. Wild-type p53 protein on cell cycle regulation has an important role in promoting DNA repair when DNA damage, or even induce cell apoptosis. Vascular endothelial growth endostatin (angiostatin, AS) on a variety of tumor growth inhibited, although it does not have a direct role in tumor cells, b