PAP on the proliferation of rat cartilage.docVIP

 PAGE \* MERGEFORMAT 13 PAP on the proliferation of rat cartilage [Abstract] Objective: To investigate the PAP (PAP) on the proliferation of cartilage cells in rats. METHODS: The rat secondary enzyme digestion of articular cartilage cells were subcultured cells and the fourth generation of drug intervention, the use of MTT colorimetric, immunohistochemical detection of proliferating cell nuclear antigen (PCNA, cell cycle detected by flow cytometry and other methods to detect the generation of times rat chondrocytes and drug intervention of the fourth generation of chondrocyte proliferation. Results: The rat chondrocytes With the passage culture, MTT proliferation than the color display decreased expression of immunocytochemical detection of PCNA decreased cell proliferation and flow cytometry showed down; PAP can inhibit this trend and promote proliferation of subcultured capacity. Conclusion: PAP on subculture of rat chondrocytes obviously promote proliferation. [Keywords:] antler polypeptides, rat, cartilage cells, proliferation Abstract Objective: The purpose of the present was to investigate the effect of pilose antler polypeptides (PAP) on the proliferation of rat chondrocyte in vitro.Methods: The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial.The 4th chondrocyte was interfered with PAP.The chondrocytes of different generations and the 4th medicine intefered generation were detected with the methods of proliferating cell nuclear antigen, MTT (methyl thiazolyl tetrazolium) assay for proliferation and Flow cytometry for analyses of cell cycle distribution and PI (proliferation index ). Results: With chondrocytes passage time increasing, the proliferating ability of chondrocytes descended.MTT assay showed that the proliferating ability of chondrocytes decreased, the expression of PCNA tapered in the experiment of immunohistochemistry, and PI descended in flow cytometry for analyses.PAP can inhibit the tenden