Participation dan ischemic preconditioning on myocardial infarct size and nitric oxide synthase protein kinase C of_0.docVIP

 PAGE \* MERGEFORMAT 16 Participation dan ischemic preconditioning on myocardial infarct size and nitric oxide synthase protein kinase C of Of: Wenqing Xiang, Yang Hongzhi, Shang Juju, Liai Yong, Wei-Hong Liu, Geng Jianguo TRADITIONAL AND [Abstract] Objective To detect the ischemic area the size of myocardial infarction, serum nitric oxide synthase (NOS) changes in protein kinase C (PKC) expression, participation element of Dan’s pharmacological preconditioning in rats. Methods Wistar rats of clean grade 60 healthy adult, male and female, body weight (274 + -42) g, mouse age in April or so, were randomly divided into 6 groups and the control group, reperfusion group, pseudo-ischemic pretreatment group, ischemic preconditioning, pharmacological preconditioning + ischemic preconditioning, pharmacological preconditioning group, n = 10. were modeling, blood, take myocardium. the cardiac tissue immersed in 10% formaldehyde solution fixation, dehydration, sealing wax, sectioned and stained. application of HE staining of histological sections were stained, 4 magnification with color pathological image analysis of myocardial infarct size. will be taken from the blood serum after centrifugation, by ultraviolet spectrophotometry serum levels of total NOS energy and vitality sub-NOS. application of protein kinase C (PKC) immunohistochemistry kit for processing tissue sections, the expression of PKC were observed 20 times. Results Participation dan can reduce myocardial infarction in rats area, and with the synergistic effect of ischemic preconditioning can enhance their ability of anti-myocardial ischemia. each group changes in serum NOS was no significant difference (Pgt; 0.05). ischemic preconditioning, pharmacological preconditioning + ischemic preconditioning and pharmacological preconditioning group, the expression of PKC significantly, other groups did not express. Conclusions Participation dan can reduce myocardial infarct size, with a pharmacological precond