Panax notoginseng saponins reverse multi-drug resistant breast cancer cells MCF-7-ADM Experimental Study.docVIP

 PAGE \* MERGEFORMAT 3 Panax notoginseng saponins reverse multi-drug resistant breast cancer cells MCF-7/ADM Experimental Study Author: Liu Lili, Liu Yane, FANG Guo-tao [Abstract] Objective To look for traditional Chinese medicine safe and effective multi-drug resistance reversal agent, and a preliminary study of Panax notoginseng saponins (PNS) on human breast cancer cell line MCF-7 / S (sensitive cells), and MCF-7/ADM (resistance Drugs cells) studied. Methods MTT determination of PNS on the MCF-7 / S and MCF-7/ADM a direct cytotoxic effect; determination of doxorubicin (DOX) on the two kinds of cells and calculate the toxic effects of multiple drug resistance; to noncytotoxic injection of PNS as a resistance reversal agents in vitro drug sensitivity by MTT test to observe the multi-drug resistant cell line MCF-7/ADM reversal effect; by RT-PCR sensitivity were measured after the cells and the role of anti-PNS Drugs cells express the MDR1 gene-positive rate; measured using WESTERN-sensitive cells and resistant cells after PNS role of multidrug resistance protein expression of P - gp (P - 170) of the positive rate. The above experiment were used as a positive control verapamil. Results ① PNS at 300μ g / ml concentration of the two cells below the basic non-toxic, safe and effective reversal dose. ② adriamycin-resistant cells to sensitive cells and the half inhibitory concentration (IC50) were 0.62μ g / ml and 25.03 μ g / ml, drug-resistant multiple of 40.37 times. ③ PNS can enhance doxorubicin (DOX) on MCF-7/ADM cytotoxicity, PNS 50,100,200 μ g / ml were applied to drug-resistant breast cancer cells 48 h after the drug-resistant cell line IC50 were reduced to 17.85 , 13.80,11.63 μ g / ml. ④ PNS (50,100,200 μ g / ml) can be reduced multidrug resistance gene mdr - 1 and expression of glycoprotein P - gp amount, and with increasing the concentration of PNS, downward effect more pronounced. But have not found to have a similar effect of verapamil. Conclusions