P21ras gene and its relationship with nasopharyngeal carcinoma research.docVIP

 PAGE \* MERGEFORMAT 10 P21ras gene and its relationship with nasopharyngeal carcinoma research [Keywords:] gene Nasopharyngeal Carcinoma Research 1.p21ras gene structure [1] Ras oncogene is to apply the genome of tumor cells transduced mouse fibroblast cells and their shape in nude mice Screening of tumor foci, one of the cloned oncogene family. Of this gene located in human chromosome 6p21.l, the gene length 85kb, respectively, with 3 the length of 68bp, 450bp, 1600bp exon, translation initiation signal located outside the second Exon, its cDNA is about 2.1kb, encoding Mr of 21x103, with 495 amino acid protein, called p2 1ras, is a nuclear protein. In mammalian cells, ras oncogene family of three structurally similar as Members, namely, Ha-ras, Ki-ras and N-ras. Three kinds of Ras protein structure of an amino acid sequence is a high-end Degree of the same, the difference is mainly the 20 carboxyl-terminal amino acid sequence. 2.p21ras function 2.1P21ras in cell signal transduction pathway plays an important role. P21ras positioned in the cell membrane is the The fundamental premise of its being activated; nucleotide exchange proteins (GEFs) promote GDP separation P21ras turn with the same GT P, forming P21ras-GTP complex, thereby activating P21ras, so that the intracellular signal transduction system to open Release. P21ras activity with GTP can be hydrolyzed GTP to GDP, p21ras deactivation combination with GDP, so that fine Intracellular signal transduction system is switched off. 2.2P21ras cancer gene mutation is an important mechanism for gene activation. Ras gene point mutation of p21 ras N-terminal glycine by other amino acids to replace the loss of a spiral, so that p21ras activity by the GTP Weak, the role of hydrolyzed GTP to GDP was inhibited, p21ras continue to work with GTP binding, so that cell division and endless Proliferation. Also found that p21ras activity of the GTP regulatory proteins by the two types o