Panax notoginseng saponins on human bone marrow hematopoietic cells in the expression of apoptosis-related proteins.docVIP

 PAGE \* MERGEFORMAT 8 Panax notoginseng saponins on human bone marrow hematopoietic cells in the expression of apoptosis-related proteins Author: Chen Xiaohong, Gao Rui-lan, Zheng Yan, Qian Xu Dai, Wei-Hong Xu Abstract This paper studies the observation of 37 saponins (PNS) on hematopoietic cells in the promotion of apoptosis-related protein (Daxx, Fas) and inhibiting apoptosis of nuclear transcription factor (NFkB, c-Rel) expression to explore the PNS to promote hematopoietic cell proliferation support the growth and survival in rats. Semi-solid colony culture of human bone marrow was observed PNS erythroid, granulocyte progenitor hematopoietic cells (CFU-GM, CFU-E) proliferation of the role of trypan blue staining cells was observed survival rate, smear staining cells was observed morphological changes, cell apoptosis by flow cytometry analysis of the situation. At the same time, dealing with the PNS granulocyte HL-60, erythroid K562, megakaryocytic CHRF-288, and Meg-01 4 cell lines, the cytoplasmic protein and nuclear protein extraction, Western blot observe Daxx, Fas, NFkB and c - Rel protein expression. The results showed that: ① PNS can stimulate human bone marrow erythroid, granulocyte progenitor cells and HL-60 and other four kinds of cell proliferation. ② HL-60 and other four kinds of cell lines treated by PNS and hunger, active good, is not observed in microscopic morphological features of apoptosis; Annexin Ⅴ analysis of apoptosis-positive cells had not been seen. ③ Western blot results showed that, after PNS treated 4-cell levels of Daxx protein expression compared with the control, respectively, decrease 33.3% -61.5%; HL-60 cells expression of Fas protein, low in itself, PNS has also decreased significantly after treatment, while the K562, CHRF-288, and Meg-01 cells, Fas protein expression compared with control, decrease 33.3% -71.4%, respectively. ④ NFkB, c-Rel protein in addition to HL-60 cells induced no significant changes in the