Potentilla discolor by UV spectrophotometry of total flavonoids content.docVIP

 PAGE \* MERGEFORMAT 7 Potentilla discolor by UV spectrophotometry of total flavonoids content [Abstract] [Objective] To investigate the turn hundred herbs in the determination of total flavonoids. [Methods] aluminum nitrate, sodium nitrite and sodium hydroxide as chromogenic reagent, the use of ultraviolet spectrophotometry Potentilla discolor the content of total flavonoids . [Results] The optimal detection wavelength of 510nm, rutin concentration in the 20 ~ 50mg / L range, absorption and mass concentration showed good linear relationship, regression equation y = 7.7457x 0.0009 (r = 0.9996) The average recovery was 99.52%, RSD 1.23%. [Conclusion] The ultraviolet spectrophotometry of total flavonoids in Potentilla discolor the content of simple, convenient, stable and reliable results. [Keywords:] herbal flavonoids with UV spectrophotometry ABSTRACT: OBJECTIVETo study assay method of total flavanone content in Potentilla discolor bunge.METHODSWith AlCl3  NaNO2  NaOH as chromogenic agent, total flavanone content of Potentilla discolor bunge is determined by ultraviolet spectrophotometry.RESULTSThe optimal detective wavelength was 510nm, and absorbability and mass concentration was satisfactory linear relationship at the mass concentration of rutin was in 20 ~ 50mg / L. The regression equation was y = 7.7457x +0.0009 (r = 0.9996), and the average recovery rate was 99.52% and RSD was 1.23%. CONCLUSIONUsing ultraviolet spectrophotometry to detect total flavanone content in Potentilla discolor bunge is simple, accurate, and sensitive. Keywords:: flavones; spectrophotometry, altravionlet; plants, medicinal Potentilla discolor (Potentilla discolor bunge) for the Rosaceae plant dry the whole plant, containing tannin, inclusive of all parts of plant flavonoids [1], with lower blood fat, anti-bacterial, diarrhea and enhanced immune function [2] . In this study, UV spectrophotometry right Potentilla discolor the content of total flavonoids were determined, aime