Propolis induced apoptosis of laryngeal carcinoma cells.docVIP

Propolis induced apoptosis of laryngeal carcinoma cells Of: Wang Juxiang, Zheng Haiping, Pei Shigeng, doors Jin E, Zhang Xiangyang [Abstract] Objective To investigate the beads of propolis on Hep-2 laryngeal carcinoma cells apoptosis and its mechanism of action. Method using flow cytometry, in situ end labeling, confocal laser microscopy, we observed inhibition of propolis on cell, withered death situations and changes in intracellular Ca2 +. Results propolis inhibited the growth of Hep-2 cells, induced apoptosis in laryngeal cancer cells, non-specific to Hep-2 cell cycle block the whole process. situ end labeling to detect apoptosis also time and dose dependent manner. propolis intracellular Ca2 + concentration increased with the increase, and with statistical significance. Conclusion propolis could inhibit the growth of laryngeal cancer cells, the inhibition of laryngeal cancer cells through induction of apoptosis play a role . [Keywords:] propolis, Hep-2 cells, apoptosis Currently, the treatment of laryngeal cancer is a comprehensive treatment based on surgery, chemotherapy is an important component of chemotherapy for laryngeal cancer is still a lack of ideal selectivity. Propolis is a bee plant buds and branches of trees collecting resin material, with its saliva into an aromatic mixture of colloidal processing of solids, containing a variety of flavonoids, quercetin, cinnamic acid derivatives, polysaccharides, amino acids, which are pharmacologically active compounds [1]. It not only has the original disease microbial, antioxidant, anti-inflammatory, liver protection effect, also has anti-tumor effect. To further explore the anticancer mechanism of action, we observed the propolis on laryngeal cancer Hep-2 cell apoptosis. are reported below. 1 Materials 1.1 Propolis extract Propolis take the property, frozen crushed soaked with 95% ethanol 72 h, filtered and the filtrate vacuum evaporated to dryness at 40 solvent, dimethyl sulfoxide (DMSO