Propolis Zuo Jin triple anti-Helicobacter pylori stomach bacteria in vitro experimental study.docVIP

Propolis Zuo Jin triple anti-Helicobacter pylori stomach bacteria in vitro experimental study Author: Li Ping Zhou Hua Chu Zhang will be more 5 Yang Liu Hongru [Abstract] Objective: In order to scientifically confirm that the left propolis gold triple anti-Helicobacter pylori stomach (HP) is effective. Methods: The orthogonal experimental design, optimization HP solid culture prescriptions fold agar dilution method compared with three kinds of drugs inhibit HP, MIC; the same time the joint susceptibility test to determine, after each combination of antimicrobial drugs and were more ring size, MIC determination ; Results: The combined use of Coptis and Evodia HP showed additive effect; berberine combined with propolis showed synergy against HP; 3 together drug combination showed synergy against HP, indicating that propolis, Coptis chinensis, Evodia three kinds of drugs in combination in vitro after application significantly enhanced the role of anti-HP, and there is dose-dependent relationship. Conclusion: Propolis Zuo Jin Weisanlian anti-HP in vitro antibacterial effective. [Keywords:] Propolis Zuo Jin Weisanlian; HP; vitro antibacterial; experimental study 1 Materials and methods 1.1 Material Propolis: from Yunnan, Ding identification of apiculture industry and trade company for sale; Sichuan Coptis, Evodia identified by the Kunming Pharmaceutical Sales Hongxiang; HP standard strain by the China Center for Disease Control and Prevention of infectious diseases prevention and control provided by. 1.2 Method [1] Preparation of Propolis Extract: that taking propolis 1.8kg, plus 5 times by weight more than 70% ethanol 9L, soaking 24h, poured filtrate; residues plus 80% ethanol 3L, soak 24h filtering; residues re - Repeat soaking filter (Method with the first two times), the merger three times the filtrate, recover ethanol, may extract 0.9kg, which is equivalent to 2kg raw medicinal herbs extract 1kg. 40 ℃ using the ice reserves. Coptis, Evodia and full side liq