Rattan tea polysaccharide AGP-3 Purification and preliminary identification of the structure of.docVIP

 PAGE \* MERGEFORMAT 12 Rattan tea polysaccharide AGP-3 Purification and preliminary identification of the structure of Author: LUO Zu-you CHEN Gen-hong Zheng Xiao-Jiang WU Mou-cheng [Abstract] Objective To Tengcha water-soluble polysaccharide purified from AGP-3 components, and a preliminary identification of its structure for the vine tea polysaccharide structure and bio-activity relationship studies lay the foundation. Methods DEAE-Sephadex A-25, Sephadex G-200 and HPLC separation and purification of the vine tea polysaccharide and by IR, GC, 1HNMR and 13CNMR analysis of the vine tea polysaccharide components of the structure of AGP-3 conducted a preliminary appraisal. Results AGP-3 weight-average molecular weight (Mw) of 1.02 * 105 Da, with the mass fraction of 57.6% neutral sugar, uronic acid 32.3%, protein 3.5%. Conclusion vine tea polysaccharide fractions AGP-3 as a protein-containing complex polysaccharides. [Keywords:] rattan tea; polysaccharide; isolation and purification; Structure Identification Isolation and Purification and Preliminary Structure Identification of Polysaccharide Fraction AGP-3 from Ampelopsis grossedentata Abstract: ObjectiveTo provide a basis for the research about the relation between the structure and its bioactivities of polysaccharides from Ampelopsis grossedentata. The polysaccharide fraction AGP-3 was obtained and analyzed preliminarily after water-soluble polysaccharides from Ampelopsis grossedentata being isolated and purified.MethodsThe water -soluble polysaccharides from Ampelopsis grossedentata were isolated and purified with DEAE-Sephadex A-25, Sephadex G-200 and HPLC, and the structure of AGP-3 was analyzed preliminarily by IR, GC, 1HNMR and 13CNMR. ResultsThe molecular average-weight ( MW) of AGP-3 was 1.02 * 105 Da, containing 57.6% neutral sugar, 32.3% uronic acid and 3.5% protein.ConclusionThe polysaccharide fraction AGP-3 from Ampelopsis grossedentata is a kind of conjugated polysaccharide containing protein