Response Surface Optimization of germ soup extraction of icariin.doc

 PAGE \* MERGEFORMAT 10 Response Surface Optimization of germ soup extraction of icariin [Abstract] Objective To optimize the soup in the spermatogenic process conditions extracted icariin. Methods response surface analysis, in order to extract the index icariin experiment to study liquid ratio, ethanol concentration, extraction time and other factors on the extraction process. The results optimized icariin alcohol extract the best conditions were: 70% plus 30 times the amount of ethanol, refluxed 1.75 h, extracted twice. spermatogenic Icariin soup 1.36 g / g. Conclusion The method is simple, good stability, suitable for industrial production applications. [Keywords:] response surface analysis, icariin, extraction, Box-Benhnken Center Combination Germ soup by the dodder, epimedium, Moreover, psoralen, Ligustrum lucidum composed of 11 herbs. The animal experiments [1] proved that germ soup rats significantly decreased serum androgen levels, increased serum estrogen, ovulation progesterone levels. After years of clinical application [2] confirmed germ soup for Chinese medicine, kidney and activating blood circulation and effective treatment of women with polycystic ovary syndrome group parties. epimedium as a prescription of herbs, Epimedium flavonoid glycosides its main active ingredients, also contains alkaloids, lignans, polysaccharides and trace elements. Modern Pharmacological studies have shown that [3,4], TFE has a hormone-like effects, can make a small female Uterus weight, E2 levels increased serum T levels increased in male mice. icariin can delay the breakdown of oocyte nests, inhibit the development of primordial follicles start to reduce the consumption of follicles, so that the original quiescent increased number of follicles [5]. TFE fine living treating polycystic ovary syndrome women one of the main active ingredient. response surface method [6] to estimate the regression method as a function of the tool, multi-factor test factor with th