Response Surface Optimization of Total Flavones Extraction Process.doc

 PAGE \* MERGEFORMAT 12 Response Surface Optimization of Total Flavones Extraction Process Of: Zhang Liming, Zhang Xia, Zheng Chuanli, Dong Xue [Abstract] Objective To study the ethanol extract of total flavonoids in three Yeh optimum conditions. Methods ethanol as solvent, using response surface design methods, the extraction of the effect of three factors that ethanol concentration (C), liquid material ratio (R), extraction time (t), central composite design experiments and mathematical models to study these factors on the extraction rate of flavonoids. Results central composite design method was established for the extraction rate and the ethanol concentration (C), liquid material ratio (R) and extraction time (t) between the mathematical model significantly, R2 = 0.950 2; the best extraction conditions were 28% ethanol (V / V), liquid ratio 22 (ml / g), extraction time 2.2 h; by extracting the number of visits, the best extraction times of 2 times. The Total Flavones under the conditions of the extraction rate of 0.30%. Conclusion validation results showed that the response surface optimization of the process is stable, reasonable and feasible. [Keywords:] Three Ye; total flavonoids; response surface optimization; extraction process Ye Radix Tetrastigmae three grapes Clover Cliff Climbing plants Tetrastigma hemsleyanum Diels et Gilg dry root, distributed in Zhejiang, Jiangxi, Fujian, Taiwan, Hubei, Guangdong, Guangxi, Sichuan, Guizhou and Yunnan provinces [1 ], with heat and detoxifying, expelling wind and phlegm, pain in the function of promoting blood circulation, mainly for the treatment of heat shock stick, pneumonia, asthma, hepatitis, rheumatism, sore throat, M. scrofulaceum, carbuncle boils boil embolism [2]. Three in the folk Ye have a good treatment for cancer and non-toxic therapeutic effect. Third Ye extract (ERT) consisting mainly of starch, sugar, flavonoids, steroidal compounds and amino acids. in acute and chronic liver injury, he