Resveratrol different antigen for T lymphocytes activation and proliferation in rats under the influence.docVIP

 PAGE \* MERGEFORMAT 13 Resveratrol different antigen for T lymphocytes activation and proliferation in rats under the influence Of: Wangyou Li, ZHAO Yan Hung, Yangxing Wu Jianhua, Wuchang school [Abstract] Objective resveratrol (RSV) antigen for the differentiation of rat T lymphocyte activation and proliferation. Methods SD-Wistar rat model of heterotopic heart transplantation model of acute rejection, isolation and host spleen lymph SD for the system of cells and adult rat cardiomyocytes in vitro mixed culture were treated with different doses of RSV and CSA interference was observed with CSA RSV mixed culture in vitro T lymphocyte proliferation following; by ELISA assay of cytokines IL-two 2. The results RSV co-cultured cells can inhibit T lymphocyte proliferation and reduce IL-2 supernatant activity, the role of strength showed significant dose dependent, with a cooperative CSA. Conclusion RSV lymphocytes in vitro immunosuppressive effect in the performance culture. [Keywords:] resveratrol; different antigen; T lymphocyte activation Resveratrol (resveratro, RSV) stilbene structure containing a non-flavonoid polyphenol compounds, mainly from the roots of Polygonum cuspidatum extract of plants, with a wide range of physiological and pharmacological effect, but on the regulation of the immune system than small, we discussed different RSV antigen stimulation of T cell activation, proliferation and differentiation inhibition. 1 Materials 1.1 Reagent and instrument resveratrol, rat IL-2 ELISA test kit, collagenase were purchased from Sigma Company; GIBCO1640 were purchased from Invitrogen Corporation; microplate reader, BMG company’s products; cell incubator, Heraeus Company products. 1.2 The donor animals, the adoption of healthy SD rats of clean grade pure lines, body mass is about 180 ~ 220g, female, purchased from Experimental Animal Center of Xi’an Jiaotong University; receptor use pure clean healthy Wistar rats, male, body mass of abou