Resveratrol in vitro and in vivo mechanism of action of anti-leukemia.docVIP

 PAGE \* MERGEFORMAT 13 Resveratrol in vitro and in vivo mechanism of action of anti-leukemia [Abstract] Objective: To investigate the resveratrol (resveratrol, Res) in vivo molecular mechanism of anti-leukemia effect. Methods: The HuT  78, Jurkat and L1210 3 Zhong leukemia cell lines with different concentrations of Res different time, the application of methyl thiazolyl tetrazolium (methyl thiazolyl tetrazolium, MTT) colorimetric assay cell proliferation, flow cytometry Detection of apoptosis, protein blot Bcl  2 and Bax protein expression, immunoprecipitation assay signal transducer and activator of transcription (signal transducers and activators of transcription, STAT) 3 and phosphorylation activity (phospho  Tyr705  STAT3, p  STAT3). BALB / c mice were randomly divided into model group and 12.5,25,50 mg / (kg * d) Res Group, subcutaneous injection of L1210 leukemia cells in the establishment of model. Reverse transcription-polymerase chain reaction (reverse transcription  polymerase chain reaction, RT  PCR) detection of leukemic mice in each group of peripheral blood T lymphocytes in interleukin-6 (interleukin  6, IL  6) gene expression, flow cytometry Detection of IL  6 protein secretion, immunohistochemistry and Western blot detection of liver activity of p  STAT3 protein expression. Results: Res can inhibit the leukemia cell HuT  78, Jurkat, L1210 growth and proliferation, induce apoptosis, while significantly down-regulated Bcl  2/Bax ratio, lower activity of p  STAT3 protein expression; Res also able to dose-dependent manner down leukemia Mouse peripheral blood T lymphocytes in IL  6 mRNA expression and cellular endocrine, while reducing the activity of mouse liver tissue levels of p  STAT3 protein expression. Conclusion: Res vivo and all have significant anti-leukemia effect, and its mechanism may be related to the IL  6 and p  STAT3 related to the impact. [Keywords:] resveratrol; apoptosis; leukemia; inter