Rh antigen stability of mPEG modification of red blood cells.docVIP

 PAGE \* MERGEFORMAT 19 Rh antigen stability of mPEG modification of red blood cells Authors: QIU Yan, investigating, and Tan Ying-Xia, Zhang Yang Pei Abstract This study aimed to explore the mPEG-modified red blood cell Rh antigen stability. Erythrocyte membrane proteins using SDSelectrophoresis analysis of mPEG-modified red cell membrane protein and mPEG with the situation, red blood cell agglutination tests and blood film 4 ℃ CPD maintenance of liquid stored blood in vitro modification of red blood cells mixed with the matching experiments, observations mPEG-modified red blood cell Rh antigens have been obscured stability. The results showed that: different preservation time of the mPEG-modified red blood cell transfusion in the simulation (ie, before and after mixed-race) in blood remained unchanged, while mPEG-modified red blood cells to keep the level of free hemoglobin in the course of normal, and mixed after incubation at 37 ℃ after the mPEG-modified free erythrocyte hemoglobin levels lower than the modified red blood cells are not mixed. A comparative analysis of PEG-specific iodine staining and Coomassie brilliant blue-dyed color map discovered a special iodine dye showing mPEG combined with the erythrocyte membrane protein band, mPEG-SPA with the erythrocyte membrane protein-binding protein molecules results in migration rate of change . mPEG-modified RBC ghost agglutination test confirmed that the modified red blood cell membrane dehiscence in the post-mPEG was still effective in masking antigens. Conclusion: mPEG-SPA, whether in red cell membrane proteins were extracted separately, or membrane damage, as well as survival after the state, are able to combine with the erythrocyte membrane protein stability. Keywords: mPEG-modified red blood cell; Rh antigen; red blood cell Rh Antigen Stability of mPEG Modified Red Blood Cells AbstractThe objective of study was to investigate the Rh antigen stability of mPEG-modified R