Ribosomal DNA ITS sequence analysis in the study of medicinal plants.docVIP

 PAGE \* MERGEFORMAT 29 Ribosomal DNA ITS sequence analysis in the study of medicinal plants Author: Zhao Huan Wu Wei Zheng You-liang PAN Hong-mei Dijuan Park [Abstract] Medicinal Plants in the nuclear rDNA is highly repetitive tandem, due to simultaneous evolutionary forces, most species of these repeat units has occurred in the homozygous or nearly homozygous. 5.8SrDNA of nuclear rDNA internal transcribed spacers ITS1 and ITS2 were divided into two parts. ITS sequence variation due to faster, able to provide a more abundant variants, and information sites have been identified in the medicinal plants, authentic research, cultivation and analysis of genetic diversity of wild medicinal plants, the lower taxa phylogenetic and classification play an important role in the study. [Keywords:] ribosomal DNA ITS area of medicinal plants Abstract: Nuclear rDNA in the medicinal plants is a tandem sequence of highly repeatable units which become homogenized or nearly homogenized in most species. The internal transcribed spacers of nuclear rDNA was divided into ITS1 and ITS2by 5.8S rDNA. As mutate quickly, ITS sequences are capable of providing many variable sites and informative sites, which plays more and more important roles in the authentications of medicinal herbs, studies on the genuineness, genetic variation analysis between cultivated and wild medicine plants, systematic development and classification researches on the lower taxon of the medicinal plants. Keywords:: Nuclear rDNA; ITS sequence; Medicinal plants People to the study of medicinal plants has a long history. With the discovery of the laws of plant genetics and application of a large number of plant secondary metabolites and results of Separation, Identification and significance of the system, and further deepen people’s understanding of medicinal plants. Modern molecular biology technology is developing rapidly, so as to form the histology, anatomy and other objective indicators for the division ba