RP-HPLC method for the determination on the consumer level film brucine and strychnine content.doc

 PAGE \* MERGEFORMAT 8 RP-HPLC method for the determination on the consumer level film brucine and strychnine content [Abstract] Objective RP-HPLC (RP-HPLC) determination of the film Pingxiao brucine and strychnine content. Methods lichrospher C18 column (4.6 mm × 250 mm, 5 μm); The mobile phase was water - acetonitrile (60:40), each containing 1 000 mL potassium dihydrogen phosphate 3.4 g, sodium lauryl sulfate 1.7 g, flow rate 1.0 mL / min, detection wavelength 254 nm, column temperature 25 ℃ . Results of brucine in the 0.102 ~ 0.918 μg, of strychnine in the range of 0.208 ~ 1.872 μg good linear relationship, correlation coefficient of 0.999 9; the average recoveries were 101.07% (RSD = 2.26%), 99.29% ( RSD = 2.79%). Conclusion The method is reliable, accurate and suitable for the film Pingxiao brucine and strychnine in the content. [Keywords:] Ping Xiao film, brucine, strychnine, HPLC Abstract: Objective To establish the RP-HPLC method for determination of Bruchine and Strychine in Pingxiao Tablet. Methods The lichrospher C18 (4.6 mm × 250 mm, 5 μm) was Used as the stationary phase. The Mobile phase consisted of water-acetonitrile ( 60:40) with 1.7 g sodium dodecylsulphate and 3.4 g KH2PO4 in 1 000 mL mixture. The Detecting wavelength was 254 nm and flow rate was 1.0 mL / min. The column temperature was 25 ℃. Result The linear ranage of Bruchine and Strychine were 0.102 ~ 0.918 μg and 0.208 ~ 1.872 μg respectively. The recovery rates of bruchine and strychnine were 101.07% (RSD = 2.26%) and 99.29% (RSD = 2.79%) respectively. Conclusion This method is realiable and accurate, the method can be applied to determination the contents of Bruchine and Strychine in Pingxiao Tablet. Keywords:: Pingxiao Tablet, Bruchine; Strychine, HPLC Pingxiao film mainly composed of turmeric, Citrus aurantium (fried with bran), Agrimony, Wulingzhi, Strychnos powder, saltpeter, dry paint, alum 8 herb composition, with qi stasis, pain Sanjie, righting cured evil of the