RUNX3 gene in gastric cancer pathogenesis and mechanism.docVIP

 PAGE \* MERGEFORMAT 12 RUNX3 gene in gastric cancer pathogenesis and mechanism Of: Huojiang Tao Li Bao-hong EXPLOSIVES [Keywords:] RUNX3 gene; gastric cancer; mechanism RUNX3 gene is a recently discovered tumor suppressor gene, which can regulate cell growth and apoptosis, signal transduction and cell biological effects of other important and complex role in transcriptional regulation, because of its downregulation in human multiple kinds of malignancies, play an important role in the development process and much attention. now RUNX3 gene in gastric cancer, the role of development and local production are reviewed. 1 RUNX3 gene and protein structural characteristics RUNX3 gene is located on human chromosome 1p36.1, total length of about 67 kb, containing the P1, P2 two promoters, exons 6 and 1 290 bp open reading frame, both inclusive of the promoter region of the transcription of a number of separate starting point [1]. RUNX3 gene transcription mainly by manipulation of promoter P2, P2 is located before exon 2, GC content (64%), there was a marked around CpG islands, so in theory than the P1 promoter more susceptible to methylation [2]. RUNX3 RUNX3 gene product protein is encoded by the @, subunit consisting of 415 amino acid residues containing heterodimer. @ Subunit contains a 128 amino acids RD (Runt domain) conserved domains, RD RUNX3 in the N-terminal, containing a S-shaped immunoglobulin fold, protein-mediated DNA binding and RUNX-mediated interactions between proteins [3, 4]. subunit of RD with the target DNA can enhance the binding force, RUNX3 protein carboxyl-terminal proline-rich and serine, in the transcriptional regulation plays an important role [5, 6]. 2 RUNX3 gene in gastric cancer, the role and mechanism of the development of Osaki et al [7] found that most of RUNX3 expression in normal gastric mucosa as part of the primary cells and G cells, but in intestinal metaplasia and cancer cells do not express. Sakakura et a