Salvia extract on the release of inhibition of extracellular HMGB1.docVIP

 PAGE \* MERGEFORMAT 12 Salvia extract on the release of inhibition of extracellular HMGB1 Of: Ting-Ting Wang Xu, Chen Wang thousands of new Ni Fangying handsome glow [Abstract] Objective: To study the Salvia endotoxin lipopolysaccharide (lipopolysaccharide, LPS activated macrophage high mobility group protein B1 (high mobility group box 1, HMGB1 release and endotoxin levels of serum HMGB1. Methods : Using different concentrations of Danshen extract processing 100 ng / ml LPS activation of cultured macrophage cell line RAW 264.7, observed supernatant HMGB1 HMGB1 mRNA expression level and cell changes, and through establishment of intraperitoneal injection of endotoxin LPS mouse model of hyperlipidemia observed endotoxemia Salvia extract serum HMGB1 levels and survival rate. cell culture supernatant and serum HMGB1 levels were detected by enzyme-linked immunoassay, cell HMGB1 mRNA expression by RT PCR detected. Results: Danshen extract significantly inhibited the LPS activated macrophages release HMGB1 and HMGB1 mRNA expression, reduced serum endotoxin levels of HMGB1 and improve survival. Conclusions: RSM could inhibit HMGB1 release and macrophage Protection of endotoxin in mice. [Keywords:] Salvia, high mobility group protein B1, macrophages, endotoxemia [Abstract] Objective: To study the effects of Salvia miltiorrhiza on the release of high mobility group box 1 (HMGB1) from endotoxin stimulated macrophages and serum HMGB1 level of endotoxemia mice. Methods: HMGB1 concentration in the culture medium and expression of HMGB1 mRNA were analysed by enzyme linked immunosorbent assay and RT PCR, respectively, after 100 ng / ml lipopolysaccharide (LPS) stimulated macrophage like RAW 264.7 cells were treated with different doses of Salvia miltiorrhiza extract. In addition, the effect of Salvia miltiorrhiza extract was investigated on serum HMGB1 level and survival rate of endotoxemia mice which were established by intraperitoneal injection of LPS. Results: