Salvia on the freeze-thaw mouse ovarian follicle survival after allogeneic transplantation and its mechanism.docVIP

Salvia on the freeze-thaw mouse ovarian follicle survival after allogeneic transplantation and its mechanism.doc

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Salvia on the freeze-thaw mouse ovarian follicle survival after allogeneic transplantation and its mechanism

 PAGE \* MERGEFORMAT 16 Salvia on the freeze-thaw mouse ovarian follicle survival after allogeneic transplantation and its mechanism Of: Lin Haiyan Qin Wu Dandan Zhu Tang Zhou Ying Xiong Chengrong [Abstract] Objective: To study the freeze-thaw Danshen injection on mouse ovarian allograft ischemic injury in hypoxia and possible mechanisms. Methods: B6C2F1 neonatal day 1 (D1) mouse ovaries, the slow speed of the financial freeze Generation after transplantation to B6C2F1 male mice 8 to 12 weeks under the kidney capsule, transplanted mice were divided into control group and the administration of SM group, after transplantation 1 d (24 h, 2 d (48 h) and 14 d recovery of graft, the superoxide dismutase (SOD and inducible nitric oxide synthase (iNOS of RT-PCR semi-quantitative determination, and 14 d after transplantation, recovery of graft do all ovarian follicles HE staining and PCNA immunohistochemistry count . Results: The time after transplantation of ovarian tissue SOD mRNA expression in SM group were higher, while the expression of iNOS mRNA is lower than the control group. 14 d after transplantation, the total recovery of all ovarian follicles and Danshen group ( 2709 + -234 than the control group (1 889 + -202, P lt;0.05, follicle survival, SM group (64.1% was significantly higher (44.7%, P lt;0.05. Conclusions: Danshen injection by upregulating SOD mRNA expression, inhibition of iNOS mRNA expression in mouse ovaries to improve freeze-thaw hypoxic ischemic allograft injury, to improve the survival rate of follicles. [Keywords:] Salvia, ovary, cryopreservation, transplantation Abstract: Objective: To explore the protective effects of danshen injection on ischemia injury in heterotopically grafted newborn mouse ovaries. Methods: Ovaries from B6C2F1 newborn mice were cryopreservation and the thawed ovaries were transplantated into kidney capsules of 8 ~ 12 w adult male mice.The mice were divided into two groups: control group and danshen group.The r

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